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Separation and kinetic characterisation of biocatalysts

Objectives


At the end of this module, the student will have understood and be able to explain (main concepts):
- the mechanisms of separation techniques usually used in biochemistry, biotechnology,
more particularly in the case of biocatalysts: techniques of precipitation
(proteins, nucleic acids), techniques of centrifugation and ultra-centrifugation,
membrane techniques (MF, UF, NF) and electrophoretic techniques;
- the bases of the measure of the reaction rate of an enzyme, the various models
allowing the description of the behaviour of an enzyme, from the simplest to the
most complex, the effect of physical and chemical parameters such as the pH or the
temperature
The student will be able to:
- choose the most suitable method of bioseparation for a given context
- to know how to use the equipment and the techniques for purification of proteins (low and medium
pressure chromatography, electrophoresis)
- to establish complex enzymatic equations of reaction rate by means of models.
- to determine experimentally the various kinetic parameters of an enzyme as well as
its optimal conditions of functioning.

Needed prerequisite

Fundamentals of Organic Chemistry, Chemistry of solutions, Structural Biochemistry, Transport phenomena and reactions in fluids, Analytical Methods

Form of assessment

The evaluation of outcome prior learning is made as a continuous training during the semester. According ot the teaching, the assessment will be different: as a written exam, an oral exam, a record, a written report, peers review...